Essential Oil Composition of Lallemantia iberica Fisch. et C.A. Mey.

Essential Oil Composition of Lallemantia iberica Fisch. et C.A. Mey.

Morteza-Semnani, Katayoun

Abstract

The essential oil of the dried flowering aerial parts of Lallemantia iberica Fisch. et C.A. Mey. (Labiatae), collected from the suburb of Larijan, north of Iran, in July 2003, was isolated by hydrodistillation and analyzed by means of GC and GC/MS. Among the 26 components identified in this oil, p-cymene (22.1%), isophytol (19.8%), T-cadinol (11.1%), 3-octanol (8.1%), caryophyllene oxide (7.4%) and terpinen-4-ol (5.7%) were the major constituents.

Key Word Index

Lallemantia iberica, Labiatae, essential oil composition, p-cymene, isophytol, T-cadinol.

Plant Name

Lallemantia iberica Fisch. & C. A. Mey. (1).

Source

The dried flowering aerial parts of Lallemantia iberica were collected in July 2003 from the suburb of Larijan, Mazandaran province, northern Iran and identified by Department of Botany, Research Center of Natural Resources of Mazandaran. A voucher specimen (No. 174) was deposited at the Herbarium of the Department of Botany, Mazandaran University of Medical Sciences.

Plant Part

The dried flowering aerial parts of L. iberica were subjected to hydrodistillation using a Clevenger-type apparatus for 5 h to yield 0.89% of yellowish oil. After preparation, the oil was subjected to GC and GC/MS analysis.

Previous Work

The species of Lallemantia have been distributed in Afghanistan, India, Iran and Turkey (1). There are approximately five native Lallemantia species in Iran (2). Lallemantia iberica Fisch. et C.A. Mey. belonging to the family Labiatae, is an aromatic plant, which grows wild in Azerbaijan, Golestan, Isfahan, Kurdestan, Mazandaran, Qazvin and Tehran provinces of Iran (3,4). According to survey of the available literature, the chemical composition of oils of L. peltata and L. royleana have been studied (5,6). A survey of the literature reveals that no previous studies on the volatile constituents of L. iberica could be found.

Present Work

GC: Gas chromatographic analysis was carried out on a Perkin-Elmer 8500 gas chromatograph with FID detector and a DB-5 capillary column (30 m x 0.25 mm, film thickness 0.25 µm). Carrier gas was He with a flow rate of 2 mL/min. The oven temperature was programmed as follows: 60°C (4 min) and then 60°-220°C at 4°C/min, injector and detector temperatures were set at 240°C.

The relative percentage of compounds was calculated from the total chromatogram by the computer.

GCMS: Hewlett Packard 6890 series operated in the EI mode at 70 eV, using a DB-5 capillary column (30 m x 0.25 mm, film thickness 0.25 µm) which was programmed as follows: 60°C (5 min) and then 60°-220°C at 4°C/min. The carrier gas was He at a flow rate of 2 mL/min.

The components of the oil were identified by their retention indices relative to C^sub 9^-C^sub 28^ n-alkanes, and by comparison of their mass spectra with those of authentic samples or with data already available in the literature (7,8).

As shown in Table I, 26 components were identified in the oil of L. iberica, which represented 100% of the total composition of the oil. p-Cymene (22.1%), isophytol (19.8%), T-cadinol (11.1%), 3-octanol (8.1%), caryophyllene oxide (7.4%) and terpinen-4-ol (5.7%) were major constituents in the oil of L. iberica.

References

1. K.R. Kirtiakar and B.D. Basu, Indian Medicinal Plants. 9, pp 2761-2763, Oriental Enterprises, Uttranchal, India (2001).

2. V. Mozaffarian, A Dictionary of Iranian plant names. p307, Farhang Moaser, Tehran, Iran (1996).

3. A.Zargari, Medicinal Plants. 4, pp 112-113, Tehran University Publications, Iran (1990).

4. K.H. Rechinger, Flora Iranica. No. 150, pp 232-239, Akademische DruckU.Verlagsanstalt, Graz-Austria (1982)

5. K.H.C. Baser, M. Kurkcuoglu and T. Ozek, Steam Volatiles of Lallemantia peltata Fisch. et Mey. from Turkey. J. Essent. Oil Res., 12, 689-690 (2000).

6. A. Ghannadi and B. Zolfaghari, Compositional Analysis of the Essential Oil of Lallemantia royleana Benin. from Iran. Flav. Fragr. J., 18, 237-239 (2003).

7. R.P. Adams, Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. pp 61-424, Allured Publ. Corp., Carol Stream, IL (2001).

8. N.W. Davies, Gas Chromatographic Retention Indices of Monoterpenes and Sesquiterpenes on Methyl Silicone and Carbowax 20M Phases. J. Chromatogr., 503, 1-24 (1990).

Katayoun Morteza-Semnani

Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

* Address for correspondence

1041-2905/06/0002-0164$14.00/2-© 2006 Allured Publishing Corp.

Received: November 2003

Accepted: May 2004

Copyright Allured Publishing Corporation Mar/Apr 2006

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