Effects of feeds containing different fats on carcass and blood parameters of broilers

Effects of feeds containing different fats on carcass and blood parameters of broilers

Ozdogan, M

SUMMARY

Effects of diets containing fats on some carcass characteristics and blood parameters of broilers were investigated. Broilers were fed one of four different isonitrogenous diets containing 6% added fat. The effect of different fat sources on the moisture, ash, and fat ratios of thigh and breast of broilers was statistically significant. The effect of different fats on serum cholesterol, high-density lipoproteins (HDL), and low-density lipoproteins (LDL) in broilers of broilers was significant, but the effect of those on triglyceride values was not significant. The effect of sex on blood parameters for cholesterol and triglyceride values was not statistically significant. However, the HDL value of males and the LDL value of females were significantly different.

Key words: oil, tallow, feed, carcass, blood, broiler

DESCRIPTION OF PROBLEM

There have been numerous reports on the effects of added fats in poultry diets. Although the effects of fats and vegetable oils have been examined on diet quality and performance of animals in the previous studies, today, direct or indirect effects of fats on animal and human health are being determined. On the basis of the previous results, the effects of fats and vegetable oils need to be examined not only for production characteristics but also for meat quality and blood parameters relative to human health. When fat-related studies on chicken are scrutinized, it is found that the fats and vegetable oils according to their energy and fatty acid characteristics affect the production [1, 2, 3, 4, 5], meat quality [6, 7, 8, 9], and blood characteristics of broilers [10, 11, 12]. When the studies were examined regarding the physiology and fat metabolism of chickens, it was shown that cholesterol values in the blood and tissue of males were higher than those of females [10, 13, 14]. It has also been reported that cholesterol in blood and tissue of broilers and rats increases with age [15, 16]. In other studies on feeding with different fat sources of broiler, it has been shown that the amount of abdominal fat in female broilers is higher than that of males [13, 17, 18].

MATERIALS AND METHODS

Experimental Design

Three hundred sixty male and female 1-dold broiler chicks (Ross strain) were used. The birds were individually wing-banded, weighed, and placed randomly in 12 floor pens (15 male and 15 female chicks per pen). There were four dietary treatments with three replicates (pens) each. The experimental treatments were formulated to meet NRC [19] recommendations for all nutrients. Analyses of metabolizable energy were made using procedures of the Association of Official Analytical Chemists [20]. The diets consisted of 6% added fat from four sources and were formulated to be isonitrogenous (Table 1). The fats used included sunflower oil (SFO), corn oil (CO), soybean oil (SO), and tallow (T). All chicks were fed with starter diets up to 21 d and then were fed with finisher diets from 22 to 42 d. The diets were fed ad libitum. Water was provided by nipple drinkers.

Data Collection

Prior to slaughter, broilers were given a feed withdrawal period of 12 h to decrease the effects of feeding on blood parameters of the chicken. Before slaughter, two male and two female chickens were randomly selected from one replicate for each of the four treatments for blood sampling and carcass analysis. Blood samples were obtained from each bird by jugular vein puncture and drawn into vacuumed capillary tubes in order to determine the blood cholesterol, triglyceride, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels. After coagulation, blood samples were centrifuged at 2,000 rpm, and then serum was collected and stored at -20[degrees]C for later analysis. Blood cholesterol, triglyceride, HDL, and LDL levels were determined spectrophotometrically [21] by using commercial kits [22]. Blood parameter values were expressed as milligrams per 100 mL.

After blood processing, four broilers were slaughtered, defeathered, processed (removal of head and feet), and eviscerated (removal of gastrointestinal tract). Carcasses were stored at 4[degrees]C for about 16 h. Afterward, the carcasses were dissected, and samples from the different portions were collected: breast meat plus attached skin, thigh muscles plus attached skin. Meat samples were homogenized using a blender with horizontal blades, and samples were frozen and stored in a freezer at -20[degrees]C until further analyses. Chemical analyses for moisture, protein, and ash were performed on all meat samples. Fat was extracted with ethyl ether using a Soxhelet apparatus [20].

Statistical Analysis

Analysis of variance of the data was conducted by using pen means. Differences among treatments were determined with Duncan’s multiple-range test. All statistical analyses were performed using SAS statistical programs [23].

RESULTS AND DISCUSSION

The effects of different fat sources added in broiler diets on broiler meat composition are shown in Table 2. The moisture content of thigh meat was significantly higher in broilers led a diet containing SO (P

The crude fat content of thighs and breasts were found to be statistically different (P

Cholesterol, triglyceride, HDL, and LDL values in serum of broilers fed with diets containing different fat sources are shown in Table 3. Cholesterol, HDL, and LDL values in blood serum of broilers fed with different fat sources were significantly different (P

All studies, however, do not agree. Fan et al. [30], for example, found that diets containing different fat sources did not affect blood cholesterol values. Although the HDL value was the highest in the groups fed with diet containing CO, this value was lowest in the groups fed with tallow added in diet. Although the LDL value was highest in groups fed with diet containing T, this value was lowest in groups fed with diet containing SFO. In the current study, HDL values were highest in the groups fed the diet containing CO and lowest in the groups fed T. Contrary to this, LDL values were highest in groups fed T and lowest in groups fed the diet containing SFO. When previous research of HDL and LDL was examined, it was concluded that the blood and products of animals had high HDL and low LDL values. On the other hand, several researchers [10, 31, 32, 33] have shown that low HDL and high LDL are values associated with atheroschiorosis. Researchers [31, 32] have also shown that increased serum HDL is able to decrease the negative effect of high blood cholesterol.

The effect of sex on HDL and LDL values was found to be statistically significant. Although HDL values were high in males (P

CONCLUSIONS AND APPLICATIONS

1. The effect of fat sources in the feed on the moisture, ash, and fat content of thigh and breast of broilers was different. The different fat sources did not have any statistical significant effect on protein content of thigh or breast meat.

2. The highest values of cholesterol, triglyceride, HDL, and LDL were in the groups fed with the diets containing CO, SFO, CO, and T, respectively.

3. The lowest values of cholesterol, triglyceride, HDL, and LDL were in the groups fed with diets containing SFO, CO, T, and SFO, respectively.

4. The amount of HDL in male chickens and the amount of LDL in female chickens were determined to be significantly higher than in the opposite sex.

5. Because of human health concerns, for example, atheroschlorosis disease, it was shown that the relative high HDL and low LDL values were positive attributes of broiler meat in the human diet.

REFERENCES AND NOTES

1. Ozdogan, M., and M. ,Sari. 2001. Fat addition to broiler diets. J. Anim. Prod. 42:28-34.

2. Farrell, D. J. 1993. One’s designer egg: Recent advances. Pages 291-302 in Recent Advances in Animal Nutrition in Australia-1993. University of New England, NSW. Australia.

3. Coon, C. N., W. A. Becker, and J. V. Spencer. 1981. The effect of feeding high energy diets containing supplemental fat on broiler weight gain, feed efficiency, and carcass composition. Poult. Sci. 60:1264-1271.

4. Kubena, L. F., F. N. Reece, J. W. Deaton, and J. D. May. 1973. The effect of dietary fat level on heat prostration of broilers. Poult. Sci. 52:1691-1693.

5. Rand, N. T., H. M. Scott, and F. A. Kummerow. 1958. Dietary fat in the nutrition of the growing chick. Poult. Sci. 37:1075-1085.

6. Simopoulos, A. P. 1999. Evolutionary aspects of omega-3 fatty acids in the food supply. Prostaglandins Leukot. Essent. Fatty Acids 60:421-429.

7. Hrdinka, C., W. Zollitsch, W. Knaus, and F. Lettner. 1996 Effects of dietary fatly acid pattern on melting point and composition of adipose tissues and intramuscular fat of broiler carcasses. Poult. Sci. 75:208-215.

8. Blanch, A., S. Lopez-Ferrer, A. C. Barroeta, and M. A. Garshorn. 1995. Effect of different dietary fat sources on cholesterol content in tissues of broiler chickens. Comparison of two methodologies. Pages 453-459 in Proc. 12th Eur. Symp. Qual. Poult. Meat, Zaragoza, Spain.

9. Bartov, I., Bianka Lipstein, and S. Bornstein. 1974. Differential effects of dietary acidulated soybean oil soapstock, cottonseed oil soapstock and tallow on broiler carcass fat characteristics. Poult. Sci. 53:115-124.

10. Oayzdogan, M. 1999. The effects of mixed fat in broiler rations on the performance and some blood parameters in summer seasons. Ph.D. Thesis. Adnan Menderes University, Aydin, Turkey.

11. Siegel, S. H., M. S. Hammad, M. R. Leach, G. F. Barbato, H. M. Green, and L. H. Marks. 1995. Dietary cholesterol and fat saturation effects on plasma esterified and unesterified cholesterol in selected lines of Japanese quail females. Poult. Sci. 74:1370-1380.

12. Miller, E. C., H. M. Menge, and C. A. Demon. 1962. Effect of dietary fat on tissue fat and plasma cholesterol level in broilers. Poult. Sci. 41:970-974.

13. Swierczewska, E., and J. Niemiec. 1995. The levels of cholesterol and lipids in the liver and muscles of 7 week old males and females fed the feed containing rapeseed “oo.” Pages 447-452. In Proc. 12th Eur. Symp. Qual. Poult. Meal, Zaragoza, Spain.

14. Demir, H., and O. Ozturkcan. 1991. The factors causing by fattening in broiler and their control. Broylerlerde yaglanmaya etki eden faktorler ve kontrolu. Anim. Mag. 5:39-45.

15. Leclercq, B. 1984. Adipose tissue metabolism and its control in birds. Poult. Sci. 63:2044-2054.

16. Kano, Y., T. Sawasaki, M. Hirayama, and K. Imai. 1977. After-effects of temperature acclimation in early life on lipid metabolism in mice in later life. Jpn. J. Vet. Sci. 39:135-140.

17. Mendes, A. A., L. H. Ancona, A. E. Laveaga, and J. G. Franco. 1995. Effect of energy:protein ratio in the diet on abdominal fat specific gravity and chemical composition of the carcass of broiler chickens. Vet. Zootec. 7:41-48.

18. Tuncer, S. D., R. Asti, B. Coskun, M. A. Tekes, and H. Erer. 1987. Effect of different energy sources on the performance and abdominal fat accumulation of broiler. J. Selcuk Univer. Vet. Fac. 3(8):25-40.

19. National Research Council. 1984. Nutrient Requirement of Domestic Animals. Nutrient Requirements of Poultry. Natl. Acad. Press, Washington, DC.

20. Association of Official Analytical Chemists. 1990. Official methods of analysis. 15th ed. Association Official Analytical Chemists, Washington, DC.

21. Ilab 900 Clinical Chemistry System equipment, Instrumentation Laboratory Company, Braunschweig, Germany.

22. IL Test Cholesterol and IL Test Triglycerides kits, Instrumentation Laboratory, Braunschweig, Germany.

23. SAS Institute. 1985. System for Elementary Statistical Analysis. 5th ed. SAS Instute, Inc., Cary, NC.

24. Sanz, M. A., A. Flores, and C. J. Lopez-Bote. 2000. The metabolic use of energy from dietary fat in broilers is affected by fatty acid saturation. Br. Poult. Sci. 41:61-68.

25. Leeson, S., J. D. Summers. 1991. Use of speciality feeds. Commercial Poultry Nutrition. Canada University Books. Ontario, Canada.

26. Hulan, H. W., F. G. Proudfoot, and D. M. Nash. 1984. The effects of different dietary fat sources on general performance and carcass fatty acid composition of broiler chickens. Poult. Sci. 63:324-332.

27. Mcleod, A. J. 1982. Nutritional factors influencing carcase fat in broilers-A review. World’s Poult. Sci. J. 38:194-200.

28. Blanch, A., and M. A. Grashorn. 1995. Effect of different dietary fat sources on general performance and carcass yield in broiler chickens. Pages 71-75. In Proc. 12th Eur. Symp. Qual. Poult. Meat, Zaragoza, Spain.

29. Verma, N. D., J. N. Panda, K. B. Singh, and A. K. Shrivastav. 1995. Effect of feeding cholesterol and fat on serum cholesterol of Japanese quail. Indian J. Poult. Sci. 30:218-223.

30. Fan, Q., J. Feng, S. Wu, K. Specht, and S. She. 1995. Nutritional evaluation of rice bran oil and a blend with corn oil. Nahrung 39:490-496.

31. Guyton, A. C., and J. E. Hall. 1996. Metabolism and temperature regulation. Pages 855-876 in Textbook of Medical Physiology. 9th ed. W. B. Saunders Company, London.

32. Bachorik, P. S., R. I. Levy, and B. M. Rifkind. 1991. Lipids and dyslipoproteinemia. Pages 188-239 in Clinical and Diagnosis Management by Laboratory Methods. J. B. Henry, ed. 18th ed. W. B. Saunders Company Inc., Philadelphia.

33. Grundy, S. M. 1991. Multifactorial etiology of hypercholesterolemia. Arterioscler. Thromb. 11:1619-1635.

Acknowledgments

This study was supported in part by Adnan Menderes University, Revolving Fund Accountancy, 09100, Aydin, Turkey.

M. Ozdogan1 and M. Aksit

Adnan Menderes University, Faculty of Agriculture, Department of Animal Science, 09100 Aydin, Turkey

1 To whom correspondence should be addressed: mozdogan@adu.edu.tr; ozdoganmursel@hotmail.com.

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