Comparative development of anurans: Using phylogeny to understand ontogeny
Maglia, Anne M
Comparative Development of Anurans: Using Phylogeny to Understand Ontogeny1
SYNOPSIS. Hypotheses of relationships are critical to describing and understanding patterns of evolution within groups of organisms. But rarely has a comparative, historical approach been employed to study developmental change, particularly among anurans. A recent resurgence of interest in collecting basic ontogenetic information provides us with the opportunity to compare ontogenetic trajectories in a phylogenetic framework. Larval skeletons and osteological development were examined for 22 taxa and compared to two hypotheses of relationships-that of Cannatella, and one proposed herein based on 41 morphological characters from larvae and 62 from adults. Larval characters were mapped on the alternate cladograms using the ACCTRAN optimization criterion. Several larval features are highly conserved among some anurans, suggesting that there is some level of canalization of morphology early in ontogeny. In contrast, a number of morphologies vary among groups, supporting the fact that there have been major evolutionary modifications to anuran larval morphologies early in ontogeny and in the early evolutionary history of anurans.
Nearly all contemporary evolutionary biologists concede that an hypothesis of phylogeny, or historical relationships, is fundamental to describing and understanding patterns of evolution within any group of organisms. Thus, in a tangible and pragmatic sense, systematics is the framework for all comparative biology. However, until recently, most studies of developmental biology (and specifically, descriptive morphological studies) were conducted in an evolutionary vacuum. Embryonic and postembryonic development of various taxa were described as stand-alone projects in efforts to document and chart staged models of development so familiar to students of vertebrate embryology (e.g., Taylor and Kollros, 1946; Patten, 1952).
Of vertebrates, anurans have been among the most common subjects of developmental studies, the results of which frequently are generalized to vertebrate systems despite the obvious morphological and developmental specializations of these amphibians. Moreover, the generalizations are based on the results of studies of a few model species chosen primarily for their hardiness as laboratory animals, such as Xenopus laevis (Nieuwkoop and Faber, 1956; Trueb and Hanken, 1992; Moon et al., 1993); Bornbina orientalis (Hanken and Hall, 1984); and Rana pipiens (Taylor and Kollros, 1946; Kemp and Hoyt, 1969). Given the limited taxonomic sampling, relatively little information can be extrapolated to describe overall patterns of postembryonic development. This is unfortunate because the skeletal reorganization experienced by anurans during metamorphosis is unparalleled among vertebrates.
A few studies of frog postembryonic development have been framed in the context of a phylogeny. Two studies (Maglia and Pugener, 1998; Trueb et al., 2000) examined the timing of ossification of elements of an individual taxon relative to a hypothesis of relationships; one other (Davies, 1989) investigated changes in timing of bone formation among a small group of frogs within the context of a cladogram. A few other studies (Haas, 1997; Larson and de Sa, 1998; Pugener et al., MS in preparation) used developmental characters to help elucidate the phylogenetic relationships of taxa.
Herein, we examine characters of the larval skeleton and patterns of anuran postembryonic development for a number of taxa within the framework of two hypotheses of relationships. By examining these taxa, and framing our results in a phylogenetic context, we gain a better understanding of the overall patterns of postembryonic development in anurans. We also determine the evolutionary history of individual morphologies, as well as identify suites of characters that may have evolved together as a result of phylogenetic, developmental, or functional constraints. Finally, we apply the results of our study to understand the evolutionary history of anurans in general.
MATERIALS AND METHODS
Larval skeleton and osteological development were examined for 22 taxa: Ascaphus truei (Ascaphidae); Bombina orientalis (Bombinatoridae); Alytes obstetricans, Discoglossus sardus (Discoglossidae); Hyla lanciformis (Hylidae); Leptodactylus fuscus (Leptodactylidae); Megophrys montana (Megophryidae); Pelobates cultripes, P. fuscus, Spea bombifrons, S. intermontana (Pelobatidae); Pelodytes punctatus (Pelodytidae); Xenopus borealis, X. laevis, X. muelleri, Silurana tropicalis, Pipa carvalhoi, P. parva, Hymenochirus boettgeri (Pipidae); Pyxicephalus adspersus (Ranidae); Rhinophrynus dorsalis (Rhinophrynidae); and Ambystoma talpoideum (Urodela: Ambystomatidae). Most data for anurans were collected from ontogenetic series staged according to the developmental table of Gosner (1960) or Nieuwkoop and Faber (for pipoids; 1956); the salamander, Ambystoma, was staged according to Wilder (1925). Specimens were cleared and double-stained for bone and cartilage following the techniques of Taylor and Van Dyke (1985), Dingerkus and Uhler (1977), or Wassersug (1976). See Appendix 1 for a list of specimens examined.
Data for seven taxa were coded from the literature, as follow: Leptodactylus fuscus, Larson and de Sa (1998); Megophrys montana, Ramaswami (1943), Sokol (1975, 1981); Pelobates fuscus, Rocek (1980); Pelodytes punctatus, Sokol (1981); Pipa carvalhoi and P. parva, Sokol (1977), and Spea intermontana, Hall and Larsen (1998). Additional data were collected from the following publications: de Beer (1937), de Sa (1988), Moore (1989), Wiens (1989), Trueb and Hanken, (1992), Pugener and Maglia (1997), Wang (1997), Maglia and Pugener (1998), de Sa and Swart (1999), Swart and de Sa (1999), and Trueb et al. (2000). Terminology is that of Gaupp (1896), Duellman and Trueb (1986), and de Sa and Trueb (1991).
A total of 41 characters (Appendices 2, 3) was coded from the larval skeletons and osteogenesis of the 22 taxa. Characters 3336 were modified from Haas (1997). Characters of the larval skeleton were coded from specimens representing the “typical” larval skeleton of each taxon-i.e., the morphology of the skeleton was relatively unchanged for at least one Gosner or Nieuwkoop and Faber stage prior, and subsequent, to the stage examined. Most of the morphological characters examined are illustrated in Figure 1.
Character evolution was examined using two similar, but slightly different methods. First, characters were optimized or “mapped” onto a cladogram of anuran relationships using MacClade 3.1.1 (Maddison and Maddison, 1992). Currently, there are several hypotheses of anuran relationships (e.g., Duellman and Trueb, 1986; Ford and Cannatella, 1993; Hay et al., 1995). Of these, the phylogeny presented by Ford and Cannatella (1993), based on morphological and life-history data, probably is the most widely accepted. Because this hypothesis is based on the separate studies of Cannatella (1985) on basal anurans and Ford (1989) on neobatrachians, we have chosen Cannatella’s hypothesis (Fig. 2A) as a framework within which to consider our data.
The second method of examining character evolution is to trace the optimizations of characters used to create a phylogenetic hypothesis. Most systematic studies of morphology, such as Cannatella’s (1985), include data derived primarily from adult specimens. However, the morphology of an organism is part of a continuous process of ontogeny that includes many different forms. Following the tenet of total evidence, because larval morphologies are considered part of the overall archetype of a taxon, they should be included in phylogenetic analyses. Therefore, we conducted an analysis of anuran relationships that included both characters coded on adult specimens (62 characters from Maglia, 2000) and the larval characters presented herein. A heuristic search was conducted using PAUP 4.0* (Swofford, 1999) with ACCTRAN optimization. All transformation series were weighed equally and unordered. Results of this analysis are presented in Figure 2B and were used as an alternate to Cannatella’s (1985) phylogeny in considering larval character evolution.’ Detailed results of the phylogenetic analysis will be discussed elsewhere (Pugener et al., MS) and the resulting phylogeny presented herein should be considered only as a framework to test hypotheses of character evolution.
Alternate phylogenetic hypotheses
The phylogenetic hypotheses considered in this investigation differ substantially in the placement of a few major groups of anurans. Cannatella (1985) recognized Discoglossidae (originally including Alytes, Barbourula, Bombina, and Discoglossus) as paraphyletic (i.e., not sharing a common ancestor). He recognized the name Bombinatoridae for the clade including Bombina (and Barbourula) and suggested that Discoglossidae is more closely related to the clade [Neobatrachia + [Pelobatoidea + Pipoidea]] than it is to Bombinatoridae. Cannatella (1985) also recognized Pipoidea as being more closely related to Pelobatoidea than either clade is to Neobatrachia. And within Pipidae, he found Silurana as the sister taxon to Pipinae (Fig. 2A).
We rooted our analysis (Fig. 2B) using the salamander Ambystoma talpoideum and found that, rather than Ascaphus, Pipoidea is the sister-taxon to all other anurans. Within Pipidae, we found Silurana and Xenopus (rather than Pipinae) to be sister taxa. Our results also suggest that Ascaphidae is the sister-taxon to the clade formed by [Discoglossidae + Bombinatoridae + [Neobatrachia + Pelobatoidea]]. Pelobatoidea and Neobatrachia (rather than Pipoidea) are sister-taxa. The combined clade [Neobatrachia + Pelobatoidea] forms an unresolved polytomy with Discoglossidae and Bombinatoridae.
The issue of outgroup inclusion is pertinent to the phylogenetic hypotheses considered herein. We included the salamander Ambystoma talpoideum as an outgroup in our phylogenetic analysis (based on the relationship Caudata [^Karaurus + Urodela] + Salientia [^Triadobatrachus + Anura] proposed by Milner, 1988, 1993; Hillis, 1991; Trueb and Cloutier, 1991; Cannatella and Hillis, 1993; Hay et al., 1995; Baez and Basso, 1996). By including this outgroup taxon, all characters coded for anurans can be completely resolved (except for those related to the suprarostral cartilages-de novo structures in anuran larvae). Because Cannatella (1985) used Ascaphus truei as the outgroup taxon in his analysis (based on the proposition that this taxon is the sistergroup to all other anurans; e.g., Cannatella and Hillis, 1993; Haas, 1997), it is impossible to resolve (or polarize) some character transformations that include Ascaphus. Of the 41 larval characters mapped onto Cannatella’s (1985) tree (Fig. 2A), Characters 12, 15, 27, and 33 are equivocal (i.e., it is not possible to determine the plesiomorphic state). However, if Ambystoma talpoideum is included as an outgroup, all the characters become fully resolved.
Because hypotheses of character evolution are dependent upon patterns of character transformations mapped onto the phylogeny, the optimization criterion utilized will affect the resulting understanding of character evolution. Throughout our discussion of character evolution, we have chosen only to discuss ACCTRAN optimizations (favoring reversals).
By optimizing each of the characters on the two phylogenetic hypotheses, we identified patterns of conserved characters (or synapomorphies), as well as other characters that seem to lack pattern and, thus, be homoplastic.
Most of the larval morphologies examined are shared by a number of taxa. Many of these can be considered shared, derived features of natural groups. In other words, they seem to have evolved in the common ancestor of monophyletic taxa.[Discoglossidae + Bombinatoridae + [Pelobatoidea + Neobatrachia]]. Five morphological features have a similar evolutionary history, as follow: divergence of the cornua trabeculae (Character 15); the otic process of the palatoquadrate (Character 26); hyobranchial spiculae (Character 35); the taenia tecti transversalis (Character 28); and the processus muscularis quadrati (Character 22). Each of these characters evolved either in the common ancestor to all frogs, with the exception of Ascaphus truei (when optimized on Cannatella’s phylogeny) or in the common ancestor to [Discoglossidae + Bombinatoridae + [Pelobatoidea + Neobatrachia]] (when optimized on the phylogeny presented herein). The difference in optimization among the two trees relates to the placement of Pipoidea.
Discoglossidae and Bombinatoridae.
One morphological feature, the presence of admandibulars (Character 12), masses of undifferentiated connective tissue lateral to the infrarostral cartilages, is found only in Discoglossidae and Bombinatoridae.[Neobatrachia + Pelobatoidea + Pipoidea]. A single morphological character, the closed dorsal margin of one side of the suprarostral cartilage (Character 4), is shared by Neobatrachia, Pelobatoidea, and Pipoidea.
Neobatrachia and Pelobatoidea. The attachment of the cornua trabeculae laterally to the suprarostral alae (Character 14) evolved in the shared ancestor of [Neobatrachia + [Pelobatoidea + Pipoidea]] when optimized on the phylogeny of Cannatella (1985). This condition reversed to form a medial attachment to the corpus of the suprarostrals in Pipoidea. If mapped on the phylogeny presented herein, lateral attachment of the cornua trabeculae is a shared, derived character for [Pelobatoidea + Neobatrachia], having evolved in the common ancestor of these taxa.
Pelobatoidea and Pipoidea. Two morphological conditions are unique to pelobatoids and pipoids-viz., fusion of middle portion of the suprarostral alae (Character 6), and medial fusion of the infrarostral cartilages (Character 10).
Neobatrachia. Two morphological conditions occur uniquely in neobatrachians. The ascending process of the palatoquadrate cartilage fuses to the braincase through what Sokol (1981) called a “high” attachment, dorsal to the oculomotor foramen (Character 25). The taenia tecti medialis (Character 29), a bar of cartilage on the dorsomedial surface of the chondrocranium that bisects the parietal portion of the frontoparietal fontanelle, evolved in the common ancestor to neobatrachians.
Pelobatoidea. Adrostral tissues (Character 9), rods of undifferentiated connective tissue lateral to the suprarostral cartilages, are present only in Pelobatoidea and seem to have evolved in the common ancestor to the group.[Pelobatidae + Pelodytidae]. A single morphological feature, lack of medial fusion of the suprarostral corpus (Character 7) evolved in the common ancestor of [Pelobatidae + Pelodytidae].
Pipoidea. Eight unique, shared-derived features occur in pipoids, as follow: position of the suprarostrals (Character 2); ethmoid plate between cornua trabeculae (Character 16); pars articularis quadrati/ posterior margin of suprarostral cartilage (Character 19); hyoquadrate process (Character 23); urobranchial (Character 34); ceratobranchials (Character 36); primordia of epipubis (Character 39); and position of the eyes (Character 40).
Pipidae. The obtuse angle of the suborbital cartilage (Character 20) and the ventrolateral process of the palatoquadrate cartilage (Character 24) are unique, derived characters shared by pipids. Five additional pipid features seem to have evolved in the ancestor to Pipoidea when optimized on Cannatella’s (1985) phylogenetic hypothesis: suprarostral alae (Character 3), barbels (Character 8), palatoquadrate (Character 18), and processus muscularis of the otic capsule (Character 32).
Xenopodinae. Four morphological features have similar evolutionary histories in the clade [Xenopus + Silurana]. These characters include the following: alae of the suprarostrals triangular (Character 3), presence of barbels (Character 8), formation of the palatoquadrate cartilage (Character 18), and processus muscularis of the otic capsule (Character 32).
Pipinae. Five unique, shared-derived characters are found in the clade [Pipa + Hymenochirus], as follow: suprarostrals reduced (Character 3); quadrato-ethmoidalis ligament (Character 21); length of frontoparietal fontanelle versus total length of chondrocranium (Character 30); size of otic capsule (Character 31); and premetamorphic fusion of first two presacral vertebrae (Character 37).
Several of the larval morphological conditions considered have an evolutionary history that includes two or more independent derivations, regardless of which phylogenetic hypothesis is considered.
Two derivations. An incomplete or open ventral margin of one side of the suprarostral cartilage (Character 5) occurs in Ascaphus, Hyla, and Leptodactylus. Mapped onto the phylogenies, this condition either (1) evolved in the ancestor of Ascaphus and closed in the common ancestor to all frogs excluding Ascaphus, or (2) evolved a second time as an open state in the common ancestor to neobatrachians and then closed in the ranid Pyxicephalus.
Similarly, a cartilaginous fusion of Meckel’s cartilage and the infrarostral cartilages (Character 11) evolved at least two times-once in the ancestor to Rhinophrynus and once in the ancestor to Hymenochirus.
Multiple derivations. Five larval morphological features seem to have evolved at least three times in the taxa considered. These are, as follow: terminally expanded cornua trabeculae (Character 17); the presence of Copula I (Character 33); the presence of an otic ligament (Character 27); the type of articulation between the cornua trabeculae and the suprarostral cartilages (Character 13); and the mode of vertebral development (Character 38).
Regardless of which hypothesis of relationships is considered, the cornua trabeculae evolved to be terminally expanded three times-in Bombina, in the common ancestor of Pelobates, and in Leptodactylus.
The presence of Copula I of the hyobranchial apparatus evolved at least four times. Optimized on Cannatella’s (1985) hypothesis, it first appeared in the common ancestor of all frogs, excluding Ascaphus. Subsequently, Copula I was lost in the common ancestor to [Neobatrachia + [Pelobatoidea + Pipoidea]], but appeared again in the common ancestor to Pelobates, in Rhinophrynus, and in Hymenochirus. Optimization on our phylogenetic hypothesis is similar, with the copula evolving in parallel in the common ancestor to [Discoglossidae + Bombinatoridae], the common ancestor to Pelobates, in Rhinophrynus, and in Hymenochirus.
The otic ligament extends from the posterolateral margin of the palatoquadrate to the otic capsule. Typically, this ligament is chondrified and is referred to as the larval otic process. Relative to Cannatella’s hypothesis (1985), a ligamentous connection evolved in the common ancestor to all anurans excluding Ascaphus, subsequently reversed to a chondrified connection in the ancestor to [Neobatrachia + [Pelobatoidea + Pipoidea]] and evolved as a ligamentous connection again in the common ancestor to Spea and in Hymenochirus. Mapped onto the phylogeny presented herein, a ligamentous connection evolved in parallel three times-in the common ancestor to [Discoglossidae + Bombinatoridae], in the common ancestor to Spea, and in Hymenochirus.
The type of articulation between the cornua trabeculae and the suprarostral cartilages has evolved several different morphologies including a cartilaginous, ligamentous, or synovial attachment. Optimizing on the tree of Cannatella (1985), basal anurans possess a cartilaginous articulation and a ligamentous attachment evolved in the common ancestor to [Discoglossidae + [Neobatrachia + [Pelobatoidea + Pipoidea]]]. Subsequently, the articulation reversed to a cartilaginous one in the common ancestor to [Pelobatoidea + Pipoidea]. The articulation evolved as a synovial joint in the common ancestor [Pelobatidae + Pelodytidae], but evolved as both cartilaginous and ligamentous in Spea. Mapping the morphologies onto our hypothesis, it seems that a ligamentous articulation evolved in the common ancestor to Discoglossidae and Neobatrachia, and at least one species of Spea. A synovial articulation seems to have evolved in the common ancestor to [Pelobatidae + Pelodytidae], but to have reversed to a cartilaginous articulation in Spea.
The way in which the vertebral centra ossify varies among taxa. In Ascaphus, Bombinatoridae, Discoglossidae, Pelobatidae, Pelodytidae, and Hyla (neobatrachian), ossification originates from only the dorsal portion of the notochordal sheath (=epichordal). In all other taxa, ossification includes the entire sheath surrounding the notochord (=perichordal; Duellman and Trueb, 1986; Maglia, 2000). Relative to Cannatella’s (1985) phylogeny, the epichordal development of the vertebral centra evolved in the common ancestor to all anurans excluding Ascaphus. The perichordal condition evolved in the common ancestor to [Pelobatoidea + Pipoidea], reversed to epichordal in the ancestor of [Pelobatidae + Pelodytidae], and then perichordy evolved again in Spea. Epichordy developed another independent origin in the common ancestor to Pipidae. Mapping on our tree, perichordy is the plesiomorphic (i.e., “primitive”) condition in anurans and epichordy seems to have evolved four different times-viz., in the ancestor of [Discoglossidae + Bombinatoridae], in the ancestor of [Pelobatidae + Pelodytidae], in the ancestor of Pipidae, and in Hyla.
By examining larval morphologies and postembryonic development in the framework of phylogenetic hypotheses of anuran relationships, we identified some patterns of character evolution. Specifically, our results suggest that most of the morphologies examined were shared by various taxonomic groups, and some taxa are characterized by as many as nine shared, derived features. Also, within some groups, larval morphologies are highly conserved-viz., there are few or no differences in the morphologies among the various species. This seems to support von Baer’s (1828) suggestion that there is some level of canalization in early ontogeny. In other words, morphologies of closely related taxa are very similar at early stages, with phenotypic differentiation appearing later in ontogeny (through additions and deletions).
In the case of pipoids, most of the apomorphic morphologies (e.g., presence of a continuous ethmoid plate, lack of mouthparts, complex ceratobranchials) are associated with an extreme degree of filter feeding. Therefore, canalization in these taxa may be the result of functional constraints associated with larval feeding. However, the morphologies (e.g., divergence of the cornua trabeculae, otic process of the palatoquadrate, hyobranchial spiculae) shared by other, more ecologically and anatomically diverse taxa do not seem to be the result of functional constraint, but rather developmental or phylogenetic constraint. But because most of these morphologies characterize more inclusive taxonomic groups (i.e., families, genera), it is not likely that they are recent occurrences.
Our results also showed that, to some degree, larval morphologies do vary among taxa. Some morphologies are unique to specific groups (e.g., “high” attachment of ascending process of the palatoquadrate, presence of taenia tech medialis in neobatrachians; presence of adrostrals in pelobatoids), suggesting that there has been a number of major evolutionary modifications to larval morphologies in early ontogeny.
Also, several morphologies seem to have evolved in parallel (e.g., ligamentous connection of otic process, terminal expansion of cornua trabeculae). This information is useful because it allows us to determine if multiple evolutionary events have given rise to similar morphologies, which may in fact, not be so similar. These data may also help us to recognize potential functional or ecological constraints (in the case of convergence), as well as to help us recognize the complexity of developmental processes. Moreover, recognition of parallelisms demonstrates that patterns of development themselves are evolving, and may provide useful insights into the homologies of adult morphologies.
Finally, we can apply the information gained from this exercise to the evolutionary history of the taxa examined. Specifically, the nature of the characters unique to pipoids gives us insight into their evolutionary history. All pipoids (except Rhinophrynus) remain aquatic as adults and have a rather uncommon appearance, compared to other anurans. These features may explain, at least in part, the historical controversy about higher-level relationships among basal anurans.
In the context of Cannatella’s (1985) phylogenetic hypothesis, the most basal anuran lineages are Ascaphidae, Bombinatoridae, and Discoglossidae-all groups that include terrestrial adults and larvae with mouthparts. Accordingly, the common ancestor to all anurans would have had a larva resembling that of Bombina, Discoglossus, or any other related taxon. Given this hypothesis of relationships, pipids are assumed to have reverted to an ontogeny reminiscent of salamanders by abandoning larval specializations associated with feeding (Cannatella, 1999).
Our hypothesis offers an alternate interpretation. Early in the evolution of anurans, two major lineages appeared-pipoids on one hand and the rest of the anurans on the other. Thus, based on outgroup comparison, the ancestor to all anurans could have had a larva that resembled the tadpole of Rhinophrynus or Xenopus, at least with regard to some of its feeding specializations. Interestingly, the basal condition of pipoid frogs proposed herein is congruent with the scheme proposed by Orton (1953, 1957) and Starrett (1973), despite the fact that their conclusions were not made in the context of a phylogenetic analysis.
By using a historical approach, we were able to determine overall patterns of conservation in larval morphology and hypothesize about evolution in early ontogeny. In a reciprocal manner, because of the fairly conserved nature of larval morphologies, developmental information should be highly useful in reconstructing hypotheses of relationships and understanding evolutionary histories, particularly among more inclusive taxonomic groups.
We are grateful to Jose E. Gonzalez (Museo Nacional de Ciencias Naturales, Madrid), Charles Myers (American Museum of Natural History), Jens Vindum (California Academy of Sciences), and Arnold Kluge (University of Michigan Museum of Zoology) for loans of specimens and permission to clear and stain ontogenetic series. Christopher Sheil, Jennifer Pramuk, and Omar Torres provided comments on an earlier version of the manuscript. Portions of this research were supported by the Panorama Society of the Natural History Museum, The University of Kansas, the Colorado Herpetological Society, and NSF DEB 9521691 to Linda Trueb.
1 From the Symposium Beyond Reconstruction: Using Phylogenies to Test Hypotheses About Vertebrate Evolution presented at the Annual Meeting of the Society for Integrative and Comparative Biology, 4-8 January 2000, at Atlanta, Georgia.
3 It has been argued that examining character evolution via optimizing on the tree they were used to build presents a circular argument. However, every character in a phylogenetic analysis represents an independent hypothesis of the evolutionary history of the group. Because phylogenetic analyses result in an hypothesis based on the most parsimonious congruence of many hypotheses of evolution (i.e., characters), examination of single characters independently does not result in circularity.
Baez, A. and N. G. Basso. 1996. The earliest known frogs of the Jurassic of South America: Review and cladistic appraisal of their relationships. Munchner Geowiss. Abh. (A) 30:131-158.
Cannatella, D. C. 1985. A phylogeny of primitive frogs (archaeobatrachians). Ph.D. Diss., The Univ. of Kansas, Lawrence, Kansas.
Cannatella, D. C. 1999. Architecture. Cranial and axial musculoskeleton. In W. McDiarmid and R. Altig (eds.), Tadpoles. The biology of anuran larvae, pp. 52-91. The University of Chicago Press, Chicago and London.
Cannatella, D. C. and D. M. Hillis. 1993. Amphibian relationships: Phylogenetic analysis of morphology and molecules. Herpetol. Monogr. 7:1-7.
Davies, M. 1989. Ontogeny of bone and the role of heterochrony in the Myobatrachine genera Uperoleia, Crinia, and Pseudophryne (Anura: Leptodactylidae: Myobatrachinae). J. Morphol. 200: 269-300.
de Beer, G. R. 1937. The development of the vertebrate skull. Oxford University Press, London.
de Sd, R. 0. 1988. Chondrocranium and ossification sequence of Hyla lanciformis. J. Morphol. 195: 345-355.
de Sa, R. 0. and C. C. Swart. 1999. Development of the suprarostral plate of pipoid frogs. J. Morphol. 240:143-153.
de SA, R. O. and L. Trueb. 1991. Osteology, skeletal development, and chondrocranial structure of Hamptophryne boliviana (Anura: Microhylidae). J. Morphol. 209:311-330.
Dingerkus, G. and L. D. Uhler. 1977. Enzyme clearing of Alcian blue stained whole small vertebrates for demonstration of cartilage. Stain Technol. 52: 229-232.
Duellman, W. E. and L. Trueb. 1986. Biology of amphibians. McGraw-Hill Book Company, New York.
Ford, L. S. 1989. The phylogenetic position of the dart-poison frogs (Dendrobatidae) among anurans. Reassessment of the neobatrachian phylogeny with commentary on complex character systems. Ph.D. Diss., The Univ. of Kansas, Lawrence, Kansas.
Ford, L. S. and D. C. Cannatella. 1993. The major Glades of frogs. Herpetol. Monogr. 7:94-117.
Gaupp, E. 1896. A. Ecker’s and R. Wiedersheim’s Anatomie des Frosches. 2 Vols. Freidrich Vieweg and Sohn, Braunschweg.
Gosner, K. L. 1960. A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16:183-190.
Haas, A. 1997. The larval hyobranchial apparatus of discoglossoid frogs: Its structure and bearing on the systematics of the Anura (Amphiba: Anura). J. Zool. Syst. Evol. Res. 53:179-197.
Hall, J. A. and J. H. Larsen. 1998. Postembryonic ontogeny of the spadefoot toad, Scaphiopus intermontanus (Anura: Pelobatoidea): Skeletal morphology. J. Morphol. 238:179-244.
Hanken, J. and B. K. Hall. 1984. Variation in timing of the cranial ossification sequence of the Oriental fire-bellied toad, Bombina orientalis (Amphibia, Discoglossidae). J. Morphol. 182:245-255.
Hay, J. M., I. Ruvinsky, S. B. Hedges, and L. R. Maxson. 1995. Phylogenetic relationships of amphibian families inferred from DNA sequences of mi
tochondrial 12S and 16S ribosomal RNA genes. Molec. Biol. Evol. 12:928-937.
Hillis, D. M. 1991. The phylogeny of amphibians: Current knowledge and the role of cytogenetics. In D. M. Green and S. K. Sessions (eds.), Amphibian cytogenetics and evolution, pp. 7-31. Academic Press, San Diego.
Kemp, N. E. and J. A. Hoyt. 1969. Sequence of ossification in the skeleton of growing and metamorphosing tadpoles of Rana pipiens. J. Morphol. 129:415-444.
Larson, P. M. and R. 0. de Sd. 1998. Chondrocranial morphology of Leptodactylus larvae (Leptodactylidae: Leptodactylinae): Its utility in phylogenetic reconstruction. J. Morphol. 238:287-306.
Maddison, W. P. and D. R. Maddison. 1992. MacClade: Analysis of phylogeny and character evolution. Sinauer Associates, Sunderland, Massachusetts.
Maglia, A. M. 2000. Phylogenetic relationships of pelobatoid frogs (Anura: Pelobatoidea). Ph.D. Diss., The Univ. of Kansas, Lawrence, Kansas.
Maglia, A. M. and L. A. Pugener. 1998. Skeletal development and adult osteology of Bombina orientalis (Anura: Bombinatoridae). Herpetologica 54: 344-363.
Milner, A. R. 1988. The relationships and origin of living amphibians. In M. J. Benton (ed.), The phylogeny and classification of the tetrapods, Vol. 1, pp. 59-102. Clarendon Press for The Systematics Association, Spec. Publ. A, Oxford.
Milner, A. R. 1993. The Paleozoic relatives of lissamphibians. Herpetol. Monogr. 7:8-27.
Moon, R. T., R. M. Campbell, J. L. Christian, L. L. Mcgrew, J. Shih, and S. Fraser. 1993. Xwnt-5A: A maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis. Development 119:97-111.
Moore, M. K. 1989. Comparative ontogeny of cranial ossification in the Spotted Salamander, Ambystoma maculatum, and the Tailed Frog, Ascaphus truei. Masters Thesis, Louisiana State University, Baton Rouge, Louisiana.
Nieuwkoop, P D. and J. Faber, 1956. Normal table of Xenopus laevis (Daudin). A systematical and chronological survey of the development from fertilized egg till the end of metamorphosis. North Holland, Amsterdam.
Orton, G. L. 1953. The systematics of vertebrate larvae. Syst. Zool. 2:63-75.
Orton, G. L. 1957. The bearing of larval evolution on some problems in frog classification. Syst. Zool. 6:79-86.
Patten, B. M. 1952. The early embryology of the chick. Blakiston Co, Philadelphia and Toronto.
Pu gener, L. A. and A. M. Maglia. 1997. Osteology and skeletal development of Discoglossus sardus (Anura: Discoglossidae). J. Morphol. 233:267-286.
Pugener, L. A., A. M. Maglia, and L. Trueb. MS. Larval characters and phylogenetic relationships of basal anurans: A value-added contribution.
Ramaswami, L. S. 1943. An account of the chondrocranium of Rana afghana and Megophrys, with a
description of the masticatory musculature of some tadpoles. Proc. Nat. Inst. Sci. India 9:43-48.
Rocek, Z. 1980. Cranial anatomy of frogs of the family Pelobatidae Stanius, 1856, with outlines of their phylogeny and systematics. Acta Univ. Carolinae, Biol. 1980:1-164.
Sokol, 0. T 1975. The phylogeny of anuran larvae: A new look. Copeia 1975:1-23.
Sokol, 0. T 1977. The free swimming Pipa larvae, with a review of pipoid larvae and pipid phylogeny (Anura: Pipidae). J. Morphol. 154:357-425.
Sokol, 0. T 1981. The larval chondrocranium of Pelodytes punctatus, with a review of tadpole chondrocrania. J. Morphol. 169:161-183.
Starrett, P H. 1973. Evolutionary patterns in larval morphology. In J. L. Vial (ed.), Evolutionary biology of anurans. Contemporary research on major problems, pp. 251-271. University of Missouri Press, Columbia.
Swart, C. C. and R. 0. de Sa. 1999. The chondrocranium of the mexican burrowing toad, Rhinophrynus dorsalis. J. Herpetol. 33:23-28.
Swofford, D. L. 1999. PA UP: Phylogenetic analysis using parsimony, Version 4.0*. Illinois Natural History Survey, Champagne.
Taylor, A. C. and J. J. Kollros. 1946. Stages in the normal development of Rana pipiens larvae. Anat. Rec. 94:7-23.
Taylor, W. and G. Van Dyke. 1985. Revised procedure for staining and clearing small fishes and other vertebrates for bone and cartilage study. Cybium 9:107-119.
Trueb, L. and R. Cloutier. 1991. A phylogenetic investigation of the inter-and intrarelationships of the Lissamphibia (Amphibia: Temnospondyli). In H.-P Schultze and L. Trueb (eds.), Origins of the higher groups of tetrapods: Controversy and consensus, pp. 223-313. Cornell University Press, Ithaca, New York.
Trueb, L. and J. Hanken. 1992. Skeletal development in Xenopus laevis (Anura: Pipidae). J. Morphol. 214:1-41.
Trueb, L., L. A. Pugener, and A. M. Maglia. 2000. Ontogeny of the bizarre: An osteological description of Pipa pipa (Anura: Pipidae), with an account of skeletal development in the species. J. Morphol. 243:75-104.
von Baer, K. E. 1828. Entwicklungsgeschichte der Thiere: Beobachtung and Reflexion. Borntrager, Konigsberg.
Wang, Y. 1997. Postcranial skeleton and development of Alytes obstetricans (Anura: Discoglossidae), with a redescription of chondrocranial and cranial morphology. Masters Thesis, The Univ. of Kansas, Lawrence, Kansas.
Wassersug, R. J. 1976. A procedure for differential staining of cartilage and bone in whole formalinfixed vertebrates. Stain Technol. 51:131-134.
Wiens, J. J. 1989. Ontogeny of the skeleton of Spea
bombifrons (Anura: Pelobatidae). J. Morphol. 202:29-51.
Wild, E. R. 1997. The ontogeny and phylogeny of ceratophryine frogs (Anura: Leptodactylidae). Ph.D. Diss., The Univ. of Kansas, Lawrence, Kansas.
Wilder, I. W. 1925. The morphology of amphibian metamorphosis. Smith College, Northampton, Massachusetts.
ANNE M. MAGLIA,2 L. ANALIA PUGENER, AND LINDA TRUEB
Division of Herpetology, Natural History Museum & Biodiversity Research Center, and Department of Ecology and Evolutionary Biology, The University of Kansas, Lawrence, Kansas 66045-7561
2 E-mail: firstname.lastname@example.org
Copyright Society for Integrative and Comparative Biology Jun 2001
Provided by ProQuest Information and Learning Company. All rights Reserved