Multilocus enzyme electrophoresis typing of clinical Helicobacter pylori Moore, John E Multilocus enzyme electrophoresis (MLEE) has been used extensively for many years in eukaryotic cell genetics and has become a standard method in evolutionary biology.1-3 Allelic variation in a structural gene can be detected using MLEE by assessing the net electrostatic charge of the polypeptide […]
Category: British Journal of Biomedical Science
Severe acquired chylomicronaemia syndrome – a challenge to the routine laboratory Mak, C Miu Lipaemia is a common interfering (spectral or chemical) factor that can complicate biochemical analysis.1,2 In patients with chylomicronaemia syndrome, gross elevations of plasma triglyceride-rich lipoproteins, particularly chylomicrons and/or very-low-density lipoproteins are found.3,4 This causes sample turbidity that can interfere with some […]
Role of Stenotrophomonas maltophilia in hospital-acquired infection Looney, W J ABSTRACT Stenotrophomonas maltophilia (previously Pseudomonas maltophilia, Xanthomonas maltophilia) is highly resistant to antibiotics. It causes infections that result in increased morbidity, but not usually mortality, in patients with weakened host defences. The increase in S. maltophilia nosocomial infections is due to the changing nature of […]
Evaluation of a distance-learning immunology and pathology module in a postgraduate biomedical science course Ryan, M T ABSTRACT An electronic presentation of materials for a distance-learning immunology and pathology module from a postgraduate biomedical science course is evaluated. Two different electronic presentation formats for the delivery of the educational material to distance learners are assessed. […]
Modified double-disc test for detection of extended-spectrum [beta]-lactamases in Escherichia coli and Klebsiella pneumoniae Kader, A A ABSTRACT This study evaluates the performance of a modified double-disc test (MDDT) for the detection of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli and Klebsiella pneumoniae. Ninety-six isolates of E. coli and 40 K. pneumoniae are […]
Protein determination in cerebrospinal fluid by protein dye-binding assay Marshall, Thomas Abstract: In this study, Coomassie brilliant blue (CBB) and pyrogallol red/molybdate (PRM) protein dye-binding assays for total protein determination in cerebrospinal fluid (CSF) are compared. Using human albumin (HA) as a protein calibrator, protein concentration in CSF samples (n = 73) ranged from 55-1960 […]
Endomysial antibody detection using human umbilical cord tissue as substrate: reactivity of cells in Wharton’s jelly Feighery, Conleth Abstract: The measurement of immunoglobulin A (IgA) endomysial antibodies is now established as an important diagnostic test in gluten-sensitive disease. Monkey oesophagus is the commonly used tissue substrate, but it has been proposed that human umbilical cord […]
Adherence mechanisms of Candida albicans Cotter, Gary Abstract: The yeast Candida albicans is an opportunistic fungal pathogen that is capable of inducing a range of superficial and systemic diseases in the immunocompromised host. Although it displays a variety of virulence factors, one the ability to adhere to host tissue – is considered essential in the […]
Assessment of murine lung tumour development: A comparison of two techniques John, Lindsay C H Abstract: Two techniques (surface photographic analysis [SPA] and microscopic tumour analysis [MTA]) are described for assessing the stage of tumour development induced by intraperitoneal 4-(methyl nitrosamino)- 1 -(3 pyridyl)-l-butanone in syngeneic A/J mice. Parameters used to assess the number and […]
Motif-dependent DNA analysis of a methicillin-resistant Staphylococcus aureus collection Cotter, L Motif-dependent DNA analysis of a methicillin-resistant Staphylococcus aureus collection* *Dedicated to the memory of David Daly. Correspondence to: Dr Seamus Fanning Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) has become a major nosocomial pathogen in recent years. Once introduced into the hospital environment, MRSA can spread […]